Soluble Human CD40 Ligand (sCD40L) ELISA
CD40 ligand, expressed on the surface of activated T-cells, basophils, and mast cells, binds to its receptor, CD40, on the surface of B-cells and stimulates B-cell proliferation, adhesion and differentiation. A soluble isoform of CD40L in the circulation, exhibiting full activity in B-cell proliferation and differentiation assays, is able to rescue B cells from apoptosis, and binds soluble CD40.
The sCD40L ELISA is a standard quantitative sandwich enzyme immunoassay, which can be used to measure sCD40L in either fresh or frozen cell culture supernates or serum (plasma samples are not suitable for this assay). The standard curve spans the range of 0.16-10 ng/mL. The limit of detection of the assay is 0.06 ng/mL. The sCD40L levels for serum samples from normal healthy donors ranged between 0.03 and 3.98 ng/mL (mean 2.13 ± 1.0 ng/mL). If samples generate values higher than the highest standard, they can be diluted and repeated. The sCD40L ELISA is for research use only, not for diagnostic or therapeutic procedures.