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Human Secretory Phospholipase A2 (sPLA2) Concentration Assay

Phospholipases A2 (PLA2) catalyze the hydrolysis of phospholipids at the sn-2 position yielding a free fatty acid and a lysophospholipid.

Secreted phospholipases A2 (sPL A2) are small enzymes that require millimolar concentrations of calcium. This enzyme family is subdivided based on structure. Different types of sPL A2 are found in the venom of some snakes (IA, IIA, IIB), pancreatic juices (IB), rat and mouse testes (IIC), placenta, synovial fluids and platelets (IIA), and in heart, lung, mast cells, and macrophages (V).

In addition to phospholipase activity, sPLA2 concentration can be measured by a standard double-antibody ‘sandwich’ enzyme-linked immunosorbent assay, specific for type IIA sPLA2 and does not cross-react with type I, IV, V, or any other inflammatory mediators. Diluted plasma or synovial fluids are appropriate samples. The standard curve spans the range of 15-1000 pg/ml. The minimum detectable concentration is 15.6 pg/ml. This assay is for research use only and not for diagnostic purposes.

 

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