Human Secretory Phospholipase A2 (sPLA2) Activity
Phospholipase A2 (PLA2) catalyzes the hydrolysis of phospholipids at the sn-2 position yielding a free fatty acid and a lysophospholipid. The release of arachidonic acid from membrane phospholipids by PLA2 is believed to be a key step in the control of eicosanoid production within the cell. The concentration of sPLA2 in plasma or serum samples can also be measured.
The sPLA2 assay provides an accurate and convenient method for measurement of sPLA2 activity. This assay uses a phosphatidylcholine substrate which is hydrolyzed by most PLA2 (e.g., bee and cobra venoms, pancreatic, etc.) with the exception of cytosolic PLA 2,3,4. Upon hydrolysis of substrate by sPLA2, free thiols are detected using DTNB.