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Soluble Human Endothelial, Leukocyte, and Platelet Selectins ELISAs

Human Endothelial-Selectin is a transmembrane glycoprotein expressed in a transitory manner only on activated endothelial cells during stimulation. Cell-surface E-Selectin is a mediator of the rolling attachment of leukocytes to the endothelium, an essential step in the extravasation of leukocytes at the site of inflammation, thereby playing a key role in the localized inflammatory response.
Soluble E-Selectin, cleaved from the surface-expressed molecule, may activate neutrophils and act as a pro-inflammatory agent. Elevated levels of sE-Selectin in serum occur in a variety of pathological conditions.

Human Leukocyte-Selectin, a cell surface glycoprotein expressed constitutively on leukocytes, plays a role in the migration of lymphocytes into peripheral lymph nodes and sites of chronic inflammation, and of neutrophils into acute inflammatory sites. Soluble L-Selectin, derived from lymphocytes and neutrophils, retains bioactivity, and at high concentrations can inhibit binding to endothelium. Levels of sL-Selectin in biological fluids may be elevated or lowered in subjects with a variety of pathological conditions.

Human Platelet-Selectin, found constitutively within endothelial cells and platelets, is a cell surface glycoprotein that acts as a receptor to support binding of leukocytes to activated platelets and endothelium. This stored P-Selectin is mobilized to the cell surface within minutes in response to a variety of inflammatory or thrombogenic agents. The soluble form of P-selectin, found in serum and plasma, may be elevated in subjects with a variety of pathological conditions.


sE selectin (1)

sL selectin (1)

sP selectin (2)


37.0 ± 14.9 ng/ml

270-1450 ng/ml

50-230 ng/ml


29.6 ± 12.6 ng/ml

270-1450 ng/ml

20-200 ng/ml

Standard curve

0.125-8.0 ng/ml

0-60 ng/ml

0-46 ng/ml

Minimum detectable

0.01 ng/ml

0.3 ng/ml

0.2 ng/ml

(1) EDTA plasma is not recommended because these are Ca2+-dependent molecules.

(2) Since sP-Selectin is released from platelets, values in serum have been found to be significantly different compared to paired plasma samples.

The selectin assays are solid phase enzyme-linked immunosorbent assays that are designed to accurately quantitate both the recombinant and natural human selectins in fresh (within 24 hours) or frozen (within 1 month) cell culture supernates, serum, plasma, or other body fluids without prior sample purification. These ELISAs are for research use only, and are not to be used for diagnostic or therapeutic procedures.


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