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Soluble Human Intercellular Adhesion Molecule-3 (sICMA-3) ELISA

Intercellular Adhesion Molecule-3 (ICAM-3) is a very interesting molecule involved in the initial immune response, thus suggesting an important role as a disease marker for a number of different indications and pathological situations.
ICAM-3 is a member of the immunoglobulin super family and functions as a ligand for the lymphocyte function-associated antigen-1 (LFA-1) which mediates lymphocyte adhesion. Three counter-receptors have been described for LFA-1, ICAM-1, 2, and 3. ICAM-3 is an integral membrane protein with five immunoglobulin-like domains that shares high homology to ICAM-1 and ICAM-2 in the extracellular region. In contrast to ICAM-1 and ICAM-2, ICAM-3 is absent on endothelia. ICAM-3 is expressed on resting lymphocytes, monocytes and neutrophils, representing the major LFA-1 ligand on these cells. The finding that adhesion of resting T lymphocytes to LFA-1 occurs primarily via ICAM-3 combined with the fact that ICAM-3 is much better expressed than other LFA-1 ligands on monocytes and resting lymphocytes implies an important role for ICAM-3 in the initiation of immune responses.

The sICAM-3 assay is an enzyme-linked immunosorbent assay for the quantitative detection of soluble ICAM-3 in cell culture supernatants, human serum, plasma, urine, amniotic fluid, bile, or other body fluids. The sICAM-3 ELISA is for research use only and not for use in diagnostic or therapeutic procedures.

The standard curve spans the range of 0-50 ng/ml and the minimum detectable level is 0.58 ng/ml. Specimens are diluted appropriately to fall within this range. The range of sICAM-3 concentration in normal healthy human plasma and serum samples is 28-73 ng/ml with a mean of 50 ± 14 ng/ml.

 

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