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Cytokines are up and downregulated and their levels in plasma are indicative of physiologic changes. In some cases the up- or down regulation of a single cytokine is of interest, in other cases a set of cytokines, growth factors and adhesion factors needs to be measured.

We offer the measurement of several single cytokines by ELISA, and two panels of cytokines, panel 1 and panel 2. These panels use a flowcytometric bead assay that renders levels of several cytokines in one measurment in a small amount of plasma.

We will be able to customize such panels based on study requirments. For planning purposes, an excellent detailed description on the world of cytokines can be found online at: Horst Ibelgaufts' COPE: Cytokines and cells Online Pathfiner Encyclopedia

Please contact us with questions on the panels that we offer as well as requests for different cytokine panels.

Click here for sample collection and shipping.

Panel 1

Type 1 and 2 Cytokines

The two types of T helper cells influence each other by the cytokines they secrete and determine the outcome of an antigenic response toward humoral or cell-mediated immunity. TH1 cells promote cell-mediated effector responses. TH2 cells are mainly helper cells that influence B-cell development and augment humoral responses such as the secretion of antibodies. IFN-gamma, can inhibit the proliferation of TH2 cells while IL-10 can suppress TH1 functions. IL-4 plays an important role in the establishment of a functional TH2 immune response.

The cytokine assay is a multiplex fluorescent bead based immunoassay for the quantitative analyte detection by flowcytometry of human Interferon-g, Interleukin-1b, Interleukin-2, Interleukin-4, Interleukin-5, Interleukin-6, Interleukin-8, Interleukin-10, Interleukin-12 p70, Tumor Necrosis Factor a and b in cell culture supernatants, serum, plasma, or other body fluids. Microspheres coated with antibodies specific to each of the analytes can be differentiated by their sizes and by their distinct spectral addresses. A fluorescently labeled second antibody mixture binds to the analytes captured by the first antibodies on the beads. A standard curve is included for each analyte in the multiplex system in the range of 0-10 ng/ml. The samples and standards are prepared in a multiwell plate such that 80 samples can be processed all together. This assay is for research use only and is not for use in diagnostic or therapeutic procedures.

The detection limit of each analyte (pg/ml):

hIL-1b

0.8

hIL-2

9.8

hIL-4

4.6

hIL-5

3.0

hIL-6

2.9

hIL-8

1.0

hIL-10

1.3

hIL-12 p70

15.5

hTNFa

2.4

hTNFb

2.1

hIFNg

0.5