Human Arginase 1 Concentration ELISA
Arginase, an enzyme that hydrolyzes arginine to ornithine and urea in the urea cycle, is expressed primarily in the liver and to some extend in the erythrocytes (type I), as well as in many extrahepatic tissues, such as brain, spinal cord, kidney, small intestine, and mammary gland (type II). Human arginase I, circulating as a homotrimer in blood, is used clinically as a liver-specific marker, which may reflect liver injury status for monitoring conditions of patients with liver disorders or pre- and postoperative conditions of partial hepatectomy patients, and may also play an important role in autoimmune inflammation.
Arginase can be measured as protein concentration by ELISA or as enzyme activity.
The Arginase assay is a standard enzyme linked immunosorbant assay that measures natural and recombinant Arginase 1 (Liver-Type). No cross-reactivity has been observed for human Arginase II. Fresh or frozen serum and cerebrospinal fluid are suitable samples for this assay. Serum samples require very careful preparation: the erythrocytes have to be spun down immediately (within a few seconds) after taking blood to avoid hemolysis and contamination of the sample with erythrocyte arginase. The range of detection range of the assay is 5-320 ng/ml, the minimum detectable limit is 0.5 ng/ml. This assay is intended for research use only and is not for diagnostic or therapeutic procedures.