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Human Erythropoeitin (Epo), the principal regulating factor of erythropoiesis, is a glycoprotein produced primarily by the kidney and is regulated by changes in oxygen availability. Hypoxia causes an increase in the level of Epo in circulation and a resultant increase in RBC production. Abnormally high concentrations of serum Epo may be observed in various pathological states including anemia, polycythemia, renal neoplasms, benign tumors, polycystic kidney disease, renal cysts, and hydronephrosis.

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The Epo ELISA is a standard sandwich enzyme immunoassay allowing for the quantitative determination of human Epo concentration in either fresh or frozen EDTA plasma or serum without prior sample purification. This ELISA is for research use only, not for diagnostic or therapeutic procedures. The standard curve spans the range of 2.5-200.0 mIU/ml and the minimum detectable level is 0.6 mIU/mL. If specimens generate values higher than the highest standard, specimens are diluted and repeated. The normal range of Epo concentration in human serum and plasma is 3 - 17 mIU/mL.