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Special Hemoglobin Tests

 

Brilliant Cresyl Blue Stain

The Brilliant Cresyl Blue (BCB) test is performed in the work up for unstable hemoglobins and is used to detect HbH inclusions. HbH is unstable and precipitates in the red blood cells, giving the appearance of small golf balls inside the cells that are observed when staining the blood film with BCB or new methylene blue. The incubation time of the blood sample with the reagents (BCB in sodium citrate media) is approximately 1 hour in a controlled temperature setting of 37oC.

 

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