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Molecular Assays

Linear Array

A multiplexed reverse dot blot method is used to detect beta globin mutations from a panel of  common beta globin variants, including Hb S, C, E, D, and O, and greater than 95% of the most common beta -thalassemia mutations.

Labeled genomic DNA is amplified and hybridized to a membrane on which multiple allele-specific probe pairs corresponding to wild type and mutant alleles are bound.  Hybridization of biotin-labeled DNA to corresponding probes is colorimetrically detected as discrete lines. Using a reference guide, genotypes are called for each of the mutations on the panel.  Immobilization of multiple probe pairs on a single strip allows simultaneous screening for multiple mutations in a single hybridization reaction. 

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Example of a Linear Array result:

LA