Clinical Diagnostics and Research tools
A multiplexed reverse dot blot method is used to detect beta globin mutations from a panel of common beta globin variants, including Hb S, C, E, D, and O, and greater than 95% of the most common beta -thalassemia mutations.
Labeled genomic DNA is amplified and hybridized to a membrane on which multiple allele-specific probe pairs corresponding to wild type and mutant alleles are bound. Hybridization of biotin-labeled DNA to corresponding probes is colorimetrically detected as discrete lines. Using a reference guide, genotypes are called for each of the mutations on the panel. Immobilization of multiple probe pairs on a single strip allows simultaneous screening for multiple mutations in a single hybridization reaction.
Example of a Linear Array result: