Clinical Diagnostics and Research tools
Hemoglobin Separation by Citrate Agar Electrophoresis (CAE)
In most cases, the diagnosis of a hemoglobin disorder can be made using complementary protein chemistry methods such as isoelectric focusing and HPLC. Additional testing using electrophoresis at both alkaline and acid pH may be used to confirm a presumptive diagnosis.
Citrate agar electrophoresis (CAE) at acid pH (6.0 to 6.2) permits separation of the major hemoglobins based on the relative mobilities of variant hemoglobins: Hb C from Hb E and Hb O, as well as Hb S from Hb D and Hb G. However, electrophoresis will not distinguish Hb E from Hb O, nor Hb D from Hb G. Hemoglobin bands are visualized by staining with amido black and acid violet (or similar stains).