Clinical Diagnostics and Research tools
Hemoglobin Separation by Cellulose Acetate Electrophoresis (CAE)
In most cases, the diagnosis of a hemoglobin disorder can be made using complementary protein chemistry methods such as isoelectric focusing and HPLC. Additional testing using electrophoresis at both alkaline and acid pH may be used to confirm a presumptive diagnosis.
Cellulose acetate electrophoresis (CAE) at alkaline pH (8.2 to 8.6) permits separation of the major hemoglobins (Hb A, Hb F, Hb S/D, Hb C/E/O-Arab, Hb G-Philadelphia) based on the relative mobilities of variant hemoglobins. Hemoglobin bands are visualized by staining with amido black and acid violet (or similar stains).